ic50 calculation software graphpad prism 8.0 Search Results


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<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
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<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
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<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
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<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
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Tecan Systems microplate reader
<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
Microplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statpoint inc statgraphics software
<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
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Vazyme Biotech Co cck-8 cell counting kit
<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
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Tecan Systems microplate detector
<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
Microplate Detector, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriginLab corp origin version 8.0
<t>HHT</t> targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by <t>MTS</t> assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).
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Image Search Results


HHT targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by MTS assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).

Journal: American Journal of Cancer Research

Article Title: The small-molecule drug homoharringtonine targets HSF1 to suppress pancreatic cancer progression

doi: 10.62347/XFJH3424

Figure Lengend Snippet: HHT targets HSF1 to inhibit pancreatic cancer cells proliferation. A. HSF1 expression assessed by western blot across 6 PC cell lines. B. HHT selectively inhibited PC cells growth with high HSF1 expression, as determined by MTS assay (n=3). C, D. HHT was less effective in the HSF1 knockdown PC cells. E. Schematic of molecular docking showing potential binding mode of HHT to HSF1 DBD. F. Cellular thermal shift assay (CETSA) of HSF1 with HHT treatment in MIAPACa-2. G. HHT bound to HSF1 and the binding affinity was quantitatively assessed by MTS experiments (n=3).

Article Snippet: Following a 72 h treatment with HHT, KRIBB11 or DTHIB exposure, 20 μL Aqueous One solution (MTS) was then added and further incubated at 37°C for 1-2 h. Cell OD values at 490 nm were measured using CellTiter-Glo (Promega, G3581) following the manufacturer’s instructions and IC 50 was calculated using GraphPad Prism 8.0.

Techniques: Expressing, Western Blot, MTS Assay, Knockdown, Binding Assay, Thermal Shift Assay